Publication Title: 
Furlanetto et al. (2018)
Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells.
Furlanetto Ana Luiza Dorigan de Matos, Cadena Silvia Maria Suter Correia, Martinez Glaucia Regina, Ferrando Beatriz, Stevnsner Tinna, Møller Ian Max
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Series Name: 
Physiologia plantarum
We evaluated the effect of global warming on Araucaria angustifolia (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high-temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40% after incubation at 30 and 37°C for 24 and 6h, respectively, while 2h at 40 and 42°C killed 95% of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2h at 42°C. The in vitro activity of enzymes of the base excision repair (BER) pathway was determined. AP endonuclease, measured in extracts from cells incubated for 2h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30 min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited by 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and 37 C, and that they are all very sensitive to 40 or 42 C. Thus, A. angustifolia will likely be vulnerable to the short-term high-temperature events associated with global warming. This article is protected by copyright. All rights reserved.
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