Publication Title: 
Zhenghong et al. (2018)
Cloning and functional analysis of five TERMINAL FLOWER1 (TFL1)/CENTRORADIALIS (CEN)-like genes from Hevea brasiliensis.
Zhenghong Bi, Tahir Ayesha T, Huang Huasun, Hua Yuwei
Publication Year: 
Series Name: 
Physiologia plantarum
Five Terminal Flower1 (TFL1) /Centroradialis (CEN)-like genes were isolated and characterized from rubber tree (Hevea brasiliensis). All genes, except HbCEN1, were found to have conserved genomic organization, characteristic of the phosphatidyl ethanolamine-binding protein (PEBP) family. Over-expression of all of them delayed flowering and altered flower architecture compared with the wild-type counterpart. In addition, since premature-flowering of the terminal bud was successfully overcome in the tfl1-1 mutant of Arabidopsis, all these genes have a potential function similar to TFL1. qRT-PCR analysis showed higher expressions of HbCEN1 and HbCEN2 in the shoot apices and stems of both immature and mature rubber trees than in reproductive organs. HbTFL1-1 and HbTFL1-2 expression was confined to roots of three-month-old seedlings and HbTFL1-3 was significantly higher in the shoot apices of these seedlings. These results suggested that HbCEN1 and HbCEN2 could be associated with development of vegetative growth, whereas HbTFL1-1, HbTFL1-2 and HbTFL1-3 seem to be mainly related with maintenance of juvenility. In addition, four of the five genes displayed variable diurnal expression, HbTFL1-1 and HbTFL1-3 being mainly expressed during the night whereas HbCEN1 and HbCEN2 showed irregular diurnal rhythms. This article is protected by copyright. All rights reserved.
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