Publication Title: 
Vieitez et al. (1992)
Title: 
Somatic embryogenesis and plantlet regeneration from cell suspension cultures of Fagus sylvatica L
Authors: 
Vieitez F. Javier, Ballester Antonio, Vieitez Ana M.
Publication Year: 
1992
Series Name: 
Plant Cell Reports
Abstract: 
Embryogenic cell suspension cultures and somatic embryos of five genotypes of beech, were obtained from aged cultures derived from immature zygotic embryos cultured on solid medium containing both 2,4-dichlorophenoxyacetic acid and N6-benzyladenine. The origin of somatic embryos was traced from single cells. Embryos remained arrested at the globular stage on liquid media, further development was achieved after plating embryogenic aggregates on Murashige and Skoog's medium with half strength major salts supplemented with glutamine and low levels of growth regulators. Cultures of different genotypes showed significant differences in maturation frequency which was not affected by the hormone treatments assayed. The frequency of conversion of embryos into platelets was low. This frequency increased after cold storage of embryos for up to 7 months.
Publication Species: 
Fagus sylvatica (European beech)
Volume: 
11
Issue: 
12
Page Numbers: 
609-613
DOI: