Publication Title: 
Dong et al. (1994)
Title: 
Growth parameters, protein and DNA synthesis of an embryogenic suspension culture of white spruce (Picea glauca)
Authors: 
Dong Jin-zhou, Dunstan David I.
Publication Year: 
1994
Series Name: 
Journal of Plant Physiology
Abstract: 
Growth parameters of a white spruce (Picea glauca) embryogenic suspension culture were characterized. Tissue fresh mass, dry mass, number of immature somatic embryos (stage 1), intracellular protein content, and total DNA accumulation showed similar growth curves over a 21-d culture period. Extracellular proteins gradually accumulated during the first 9 d, then dramatically increased. Immature embryo number showed a positive correlation to intracellular protein and DNA accumulations, correlation coefficients for these parameters were higher than for fresh dry masses. The results from in vivo pulse-labelling with 35S-methionine showed a peak for incorporation into proteins at day 7. The protein profiles of intracellular proteins were greatly different from those of extracellular proteins.
Publication Species: 
Picea glauca (white spruce)
Volume: 
144
Issue: 
2
Page Numbers: 
201-208
DOI: 
PubMed: