Publication Title:
Bommineni et al. (1993)
Title:
Transformation of white spruce (Picea glauca) somatic embryos by microprojectile bombardment
Publication Year:
1993
Series Name:
Plant Cell Reports
Abstract:
Cotyledonary somatic embryos of white spruce (Picea glauca (Moench) Voss) were subjected to microprojectile bombardment with a gene construct containing a gus::nptII fusion gene. Somatic embryos were used to re-induce the embryogenic tissue after bombardments. Histochemical assay using X-gluc as a substrate showed that all the embryos (100%) were GUS positive 48 h after bombardment. However, only thirteen out of 605 embryos (2.2%) remained GUS positive after two months in culture. Three of those thirteen (23%) embryo-derived tissues consistently showed GUS activity for eight months in culture. These putatively transformed embryogenic tissues were subjected to Southern blot analysis and the results suggested integration of the gus::nptII gene expression cassette in the white spruce genome.
Publication Species:
Picea glauca (white spruce)
Volume:
13
Issue:
1
Page Numbers:
17-23