Publication Title: 
Kuzmin et al. (2019)
Title: 
Stepwise large genome assembly approach: a case of Siberian larch (Larix sibirica Ledeb).
Authors: 
Kuzmin Dmitry A, Feranchuk Sergey I, Sharov Vadim V, Cybin Alexander N, Makolov Stepan V, Putintseva Yuliya A, Oreshkova Natalya V, Krutovsky Konstantin V
Publication Year: 
2019
Series Name: 
BMC bioinformatics
Abstract: 
De novo assembling of large genomes, such as in conifers (~ 12-30 Gbp), which also consist of ~ 80% of repetitive DNA, is a very complex and computationally intense endeavor. One of the main problems in assembling such genomes lays in computing limitations of nucleotide sequence assembly programs (DNA assemblers). As a rule, modern assemblers are usually designed to assemble genomes with a length not exceeding the length of the human genome (3.24 Gbp). Most assemblers cannot handle the amount of input sequence data required to provide sufficient coverage needed for a high-quality assembly.
Keywords: 
  • Arabidopsis genetics
  • Genome, Plant
  • Larix genetics
  • Prunus genetics
  • Sequence Analysis, DNA methods
  • Time Factors
Volume: 
20
Issue: 
Suppl 1
Page Numbers: 
37
PubMed: