Publication Title: 
Xie et al. (2019)
Title: 
A reference-grade wild soybean genome.
Authors: 
Xie Min, Chung Claire Yik-Lok, Li Man-Wah, Wong Fuk-Ling, Wang Xin, Liu Ailin, Wang Zhili, Leung Alden King-Yung, Wong Tin-Hang, Tong Suk-Wah, Xiao Zhixia, Fan Kejing, Ng Ming-Sin, Qi Xinpeng, Yang Linfeng, Deng Tianquan, He Lijuan, Chen Lu, Fu Aisi, Ding Qiong, He Junxian, Chung Gyuhwa, Isobe Sachiko, Tanabata Takanari, Valliyodan Babu, Nguyen Henry T, Cannon Steven B, Foyer Christine H, Chan Ting-Fung, Lam Hon-Ming
Publication Year: 
2019
Series Name: 
Nature communications
Abstract: 
Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Here we report a reference-grade genome of wild soybean accession W05, with a final assembled genome size of 1013.2 Mb and a contig N50 of 3.3 Mb. The analytical power of the W05 genome is demonstrated by several examples. First, we identify an inversion at the locus determining seed coat color during domestication. Second, a translocation event between chromosomes 11 and 13 of some genotypes is shown to interfere with the assignment of QTLs. Third, we find a region containing copy number variations of the Kunitz trypsin inhibitor (KTI) genes. Such findings illustrate the power of this assembly in the analysis of large structural variations in soybean germplasm collections. The wild soybean genome assembly has wide applications in comparative genomic and evolutionary studies, as well as in crop breeding and improvement programs.
Keywords: 
  • Biological Evolution
  • DNA Copy Number Variations
  • Domestication
  • Genome, Plant genetics
  • Genomics methods
  • Genotype
  • Molecular Sequence Annotation
  • Peptides genetics
  • Plant Breeding methods
  • Plant Proteins genetics
  • Quantitative Trait Loci genetics
  • Soybeans genetics
  • Translocation, Genetic genetics
Volume: 
10
Issue: 
1
Page Numbers: 
1216
PubMed: