Publication Title: 
Maruyama et al. (2019)
Title: 
Progress in Somatic Embryogenesis of Japanese Pines.
Authors: 
Maruyama Tsuyoshi E, Hosoi Yoshihisa
Publication Year: 
2019
Series Name: 
Frontiers in plant science
Abstract: 
Somatic embryogenesis (SE) in not only one of the most promising techniques for mass propagation of selected trees, but also is a valuable tool for basic research studies in cell biology and genetic engineering, and it allows the long-term ex situ conservation of genetic resources by cryopreservation techniques. This review reports the most recent progress in SE, protoplast culture, and cryopreservation of four important Japanese pines (Pinus thunbergii, Pinus densiflora, Pinus armandii var. amamiana, and Pinus luchuensis). Induction of embryogenic tissues (ET), embryogenic culture maintenance/proliferation, production of somatic embryos, germination, and conversion to plants are described focusing on the protocols most commonly reported for plant production in Pinus species through to SE.
Volume: 
10
Issue: 
Page Numbers: 
31
PubMed: