Publication Title: 
Jiao et al. (2019)
Title: 
Cyclic GMP mediates abscisic acid-stimulated isoflavone synthesis in soybean sprouts.
Authors: 
Jiao Caifeng, Gu Zhenxin
Publication Year: 
2019
Series Name: 
Food chemistry
Abstract: 
The influences of abscisic acid (ABA)-guanosine 3',5'-cyclic monophosphate (cGMP) on UV-B treatment-stimulated isoflavone synthesis in soybean sprouts was explored. It turned out that ABA, with cGMP, up-regulated gene expression and activity of chalcone synthase (CHS) and isoflavone synthase (IFS), and subsequently induced isoflavone biosynthesis under UV-B treatment. Furthermore, data obtained from the isobaric tags for relative and absolute quantification (iTRAQ) analysis showed that there were two core components in ABA response: SNF1-related protein kinase (SnRK) and type 2C protein phosphatase (PP2C), were up and down regulated after UV-B treatment, respectively. UV-B exposure stimulated increment in guanine nucleotide-binding protein and calreticulin expression. Additionally, CHS and IFS protein expression were up regulated under UV-B stress. Overall, UV-B-induced ABA resulted in PP2C inhibition and SnRK2 activation, and up-regulated CHS and IFS expression, leading to enhancement of isoflavone accumulation. cGMP and calreticulin as downstream messengers, mediated ABA-stimulated isoflavone biosynthesis after UV-B exposure.
Keywords: 
  • Abscisic Acid pharmacology
  • Acyltransferases metabolism
  • Cyclic GMP metabolism
  • Gene Expression Regulation, Plant drug effects
  • Gene Expression Regulation, Plant radiation effects
  • Isoflavones biosynthesis
  • Protein-Serine-Threonine Kinases metabolism
  • Soybeans drug effects
  • Soybeans metabolism
  • Soybeans radiation effects
  • Ultraviolet Rays
Volume: 
275
Issue: 
Page Numbers: 
439-445
PubMed: