Publication Title: 
Wu et al. (2020)
Overexpression of the GbF3'H1 gene enhanced the epigallocatechin, gallocatechin and catechin contents in transgenic Populus.
Wu Yaqiong, Wang Tongli, Xin Yue, Wang Guibin, Xu Li-An
Publication Year: 
Series Name: 
Journal of agricultural and food chemistry
Ginkgo biloba L. leaves are a flavonoid resource for the pharmaceutical industry. The flavonoid 3'-hydroxylase (F3'H) is a key enzyme in the flavonoid biosynthesis pathway. However, the role of F3'H in flavonoid biosynthesis and metabolism is unclear. In this study, we characterized and functionally analyzed the ginkgo F3'H gene GbF3'H1 that encodes a protein of 520 amino acids. Expression profiling showed that GbF3'H1 was highly expressed in the leaves of ginkgo in September. Subcellular localization showed that GbF3'H1 occurred predominately in the cytoplasm. Transgenic poplars overexpressing GbF3'H1 had more red-colored pigmentation in leaves than did wild-type (WT) plants. Furthermore, the concentrations of epigallocatechin, gallocatechin and catechin in the downstream products synthesized by flavonoids were significantly higher in the transgenic plants than in the WT plants. These results indicate that overexpression of GbF3'H1 enhances flavonoid production in transgenic plants and provide new insights into flavonoid biosynthesis and metabolism.
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